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A perspective view on the nanomotion detection of living organisms and its features
Author(s) -
Venturelli Leonardo,
Kohler AnneCéline,
Stupar Petar,
Villalba Maria I.,
Kalauzi Aleksandar,
Radotic Ksenija,
Bertacchi Massimiliano,
Dinarelli Simone,
Girasole Marco,
Pešić Milica,
Banković Jasna,
Vela Maria E.,
Yantorno Osvaldo,
Willaert Ronnie,
Dietler Giovanni,
Longo Giovanni,
Kasas Sandor
Publication year - 2020
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2849
Subject(s) - nanotechnology , atomic force microscopy , electronics , cantilever , yeast , microscope , key (lock) , computer science , biochemical engineering , biology , materials science , medicine , engineering , electrical engineering , computer security , pathology , biochemistry , composite material
The insurgence of newly arising, rapidly developing health threats, such as drug‐resistant bacteria and cancers, is one of the most urgent public‐health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro‐fabricated cantilever and by monitoring its displacements with an atomic force microscope‐based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life‐threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.