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Molecular toxicity of nanoplastics involving in oxidative stress and desoxyribonucleic acid damage
Author(s) -
Zheng Tongtong,
Yuan Dong,
Liu Chunguang
Publication year - 2019
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2804
Subject(s) - superoxide dismutase , chemistry , oxidative stress , dna damage , quenching (fluorescence) , circular dichroism , biochemistry , gel electrophoresis , dna , fluorescence , biophysics , toxicity , organic chemistry , biology , physics , quantum mechanics
Microplastic pollution attracted extensive attention because of its global presence and adverse effects on ecosystem. However, it is insufficient to clear the effects of nanoplastics on organisms at the molecular level. Herein, a nanopolystyrene (50 nm) was used to examine molecular responses of superoxide dismutase (SOD) and desoxyribonucleic acid (DNA) using spectroscopy (UV‐vis, circular dichroism spectra, and fluorescence measurements) and single cell gel electrophoresis methods. Results showed that nanopolystyrene induced oxidative stress, involving in the increase of SOD activity and malondialdehide (MDA) content, and DNA damage because of the significant increase of olive tail moment, head optical density, and tail DNA percentage in the groups at exposure concentrations above 5 × 10 −6 mol/L. The second structural and microenvironment of aromatic amino acids of SOD were changed with nanopolystyrene exposure. The fluorescence of SOD was quenched by nanopolystyrene at exposure concentration above 1 × 10 −5 mol/L, and the quenching mode could be ascribed to the static type. The results and the combined methods are favorable to explore the molecular toxicity of other nanoplastics and the interaction mechanism.