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Nitrogen and sulfur co‐doped carbon nanodots toward bovine hemoglobin: A fluorescence quenching mechanism investigation
Author(s) -
Wang Jiajun,
Xiang Xueqing,
Milcovich Gesmi,
Chen Jingru,
Chen Chao,
Feng Jiuju,
Hudson Sarah P.,
Weng Xuexiang,
Ruan Yongming
Publication year - 2019
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2761
Subject(s) - quenching (fluorescence) , fluorescence , nanodot , photochemistry , sulfur , carbon fibers , quantum yield , materials science , chemistry , nanotechnology , organic chemistry , composite number , physics , quantum mechanics , composite material
A deep understanding of the molecular interactions of carbon nanodots with biomacromolecules is essential for wider applications of carbon nanodots both in vitro and in vivo. Herein, nitrogen and sulfur co‐doped carbon dots (N,S‐CDs) with a quantum yield of 16% were synthesized by a 1‐step hydrothermal method. The N,S‐CDs exhibited a good dispersion, with a graphite‐like structure, along with the fluorescence lifetime of approximately 7.50 ns. Findings showed that the fluorescence of the N,S‐CDs was effectively quenched by bovine hemoglobin as a result of the static fluorescence quenching. The mentioned quenching mechanism was investigated by the Stern‐Volmer equation, temperature‐dependent quenching, and fluorescence lifetime measurements. The binding constants, number of binding sites, and the binding average distance between the energy donor N,S‐CDs and acceptor bovine hemoglobin were calculated as well. These findings will provide for valuable insights on the future bioapplications of N,S‐CDs.