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DNA aggregation induced by Mg 2+ ions under different conditions
Author(s) -
Bui VanChien,
Nguyen ThiHuong
Publication year - 2018
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2721
Subject(s) - dna , ion , oligonucleotide , atomic force microscopy , chemistry , crystallography , analytical chemistry (journal) , materials science , nuclear chemistry , nanotechnology , chromatography , biochemistry , organic chemistry
Cations‐induced DNA aggregation can modify the local structure of oligonucleotides and has potential applications in medicine and biotechnology. Here, we used atomic force microscopy to investigate λ‐DNA aggregation on Mg 2+ ‐treated glass (Mg 2+ /glass) and in Mg 2+ solution. Atomic force microscopy topography images showed that some DNA fragments were slightly stacked together on 10 mM Mg 2+ /glass and stacked stronger on ≥50 mM Mg 2+ /glass. They also showed that DNA aggregated stronger in Mg 2+ solution than on Mg 2+ /glass, ie, DNAs are strongly stacked and twisted at 10 mM Mg 2+ , rolled together at 50 mM Mg 2+ , and slightly aggregated to form small particles at 100 mM Mg 2+ . At a specific condition, ie, heating λ‐DNA to 92°C, cooling down to 75°C, adding Mg 2+ , and vortexing the resulting solution, DNA strongly aggregated and formed pancake‐like shapes at 10 and 50 mM or a large aggregate at 100 mM Mg 2+ solutions. Our results may be helpful for medical applications and gene therapy using cation‐DNA technology.