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Fluorescent competitive aptasensor for detection of aflatoxin B 1
Author(s) -
Mukherjee Monali,
Bhatt Praveena,
H.K. Mamani
Publication year - 2017
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2650
Subject(s) - aflatoxin , aptamer , detection limit , chromatography , mycotoxin , linear range , chemistry , biosensor , pepper , food science , biology , microbiology and biotechnology , biochemistry
Aflatoxin B1 (AFB 1 ) is one of the most commonly found mycotoxins in food commodities, particularly cereals, oilseeds, spices and tree nuts. In the past decade, aptamers have come into limelight and emerged as a new biosensing element replacing antibodies in various detection formats. Herein we report a faster, more sensitive, high throughput method for the detection of AFB 1 using AFB 1 ‐specific aptamers. The assay format was based on a competitive reaction of the fluorescent tagged aptamer specific to AFB 1 with the aflatoxin conjugate. Under optimal conditions, a linear range of detection (50 ng to 50 pg) was achieved with a limit of detection (LOD) of 10 pg/mL in the buffer system. Results of inter‐ and intra‐assay revealed that the assay was repeatable with standard deviation in acceptable range. The assay was also validated in food samples such as dried red chilies, groundnut and whole pepper with recovery in the range of 92 to 102% at 10 ng/mL and 100 pg/mL levels. The aptasensor assay was also compared with standard analytical method of HPLC and was found to be more sensitive. This detection technique has the potential to be developed into a biosensor platform for AFB 1 detection.