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Development of highly sensitive and selective antibodies for the detection of the explosive pentaerythritol tetranitrate (PETN) by bioisosteric replacement
Author(s) -
Hesse Almut,
Biyikal Mustafa,
Rurack Knut,
Weller Michael G.
Publication year - 2016
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2511
Subject(s) - pentaerythritol tetranitrate , chemistry , detection limit , explosive material , chromatography , trinitrotoluene , organic chemistry
An improved antibody against the explosive pentaerythritol tetranitrate (PETN) was developed. The immunogen was designed by the concept of bioisosteric replacement, which led to an excellent polyclonal antibody with extreme selectivity and immunoassays of very good sensitivity. Compounds such as nitroglycerine, 2,4,6‐trinitrotoluene, 1,3,5‐trinitrobenzene, hexogen (RDX), 2,4,6‐trinitroaniline, 1,3‐dinitrobenzene, octogen (HMX), triacetone triperoxide, ammonium nitrate, 2,4,6‐trinitrophenol and nitrobenzene were tested for potential cross‐reactivity. The detection limit of a competitive enzyme‐linked immunosorbent assay was determined to be around 0.5 µg/l. The dynamic range of the assay was found to be between 1 and 1000 µg/l, covering a concentration range of three decades. This work shows the successful application of the bioisosteric concept in immunochemistry by exchange of a nitroester to a carbonate diester. The antiserum might be used for the development of quick tests, biosensors, microtitration plate immunoassays, microarrays and other analytical methods for the highly sensitive detection of PETN, an explosive frequently used by terrorists, exploiting the extreme difficulty of its detection. Copyright © 2015 John Wiley & Sons, Ltd.

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