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Preparation and rebinding properties of protein‐imprinted polysiloxane using mesoporous calcium silicate grafted non‐woven polypropylene as matrix
Author(s) -
Kan Bohong,
Feng Lingzhi,
Zhao Kongyin,
Wei Junfu,
Zhu Dunwan,
Zhang Linhua,
Ren Qian
Publication year - 2016
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2455
Subject(s) - materials science , polypropylene , fourier transform infrared spectroscopy , chemical engineering , silane , silanes , scanning electron microscope , mesoporous material , calcium silicate , adsorption , nuclear chemistry , polymer chemistry , chemistry , composite material , organic chemistry , engineering , catalysis
Calcium silicate particle containing mesoporous SiO 2 (CaSiO 3 @SiO 2 ) was grafted on the surface of non‐woven polypropylene. The PP non‐woven grafted calcium silicate containing mesoporous SiO 2 (PP‐g‐CaSiO 3 @SiO 2 ) was used as the matrix to prepare bovine serum albumin (BSA) molecularly imprinted polysiloxane (MIP) by using silanes as the functional monomers and BSA as the template. PP non‐woven grafted BSA‐imprinted polysiloxane (PP‐g‐CaSiO 3 @SiO 2 MIP) was characterized by scanning electron microscope (SEM), Fourier transform infrared spectometry (FTIR) and drilling string compensator (DSC). Influence factors on the rebinding capacity of the MIP were investigated, such as grafting degree, the pH in treating CaSiO 3 and the type and proportion of silanes. The rebinding properties of BSA on PP‐g‐CaSiO 3 @SiO 2 and MIP were investigated under different conditions. The results indicated that the rebinding capacity of MIP for BSA reached 56.32 mg/g, which was 2.65 times of NIP. The non‐woven polypropylene grafted BSA‐imprinted polysiloxane could recognize the template protein and the selectivity factor ( β ) was above 2.4 when using ovalbumin, hemoglobin and γ ‐globulin as control proteins. The PP‐g‐CaSiO 3 @SiO 2 MIP has favorable reusability. Copyright © 2015 John Wiley & Sons, Ltd.