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Spectroscopic studies on the interaction mechanisms of safranin T with herring sperm DNA using acridine orange as a fluorescence probe
Author(s) -
Long Jun,
Wang Xingming,
Xu Dongling,
Ding Lisheng
Publication year - 2014
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2341
Subject(s) - acridine orange , dna , chemistry , fluorescence , binding constant , acridine , safranin , enthalpy , stereochemistry , analytical chemistry (journal) , binding site , biochemistry , biology , chromatography , organic chemistry , thermodynamics , genetics , staining , apoptosis , physics , quantum mechanics
Under the condition of physiological pH environment (pH = 7.40), the interactions of safranin T (ST) with herring sperm DNA were studied by means of spectral methods using acridine orange (AO) as a fluorescence probe. The spectroscopic characteristics of DNA–AO in the case of ST (along with the increase of concentration) were observed in an aqueous medium. The binding constants for ST stranded DNA and competitive bindings of ST interacting with DNA–AO systems were examined by fluorescence spectra, and the binding mechanism of ST with DNA was researched via viscosity measurements. All the testimony manifested that bonding modes between ST and DNA were evidenced to be intercalative binding and electrostatic binding, and the combining constant of ST with DNA was obtained. The binding of ST to DNA was driven by entropy and enthalpy through the calculated thermodynamic parameters (Δ r H m Ө , Δ r S m and Δ r G m Ө ). Copyright © 2014 John Wiley & Sons, Ltd.