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Effect of CoCl 2 on the content of different metals and a relative activity of DNA‐hydrolyzing abzymes in the blood plasma of mice
Author(s) -
Legostaeva Galina A.,
Zaksas Nataliya P.,
Gluhcheva Yordanka G.,
Sedykh Sergey E.,
Madzharova Maria E.,
Atanassova Ni.,
Buneva Valenti.,
Nevinsky Georgy A.
Publication year - 2013
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2217
Subject(s) - ethylenediaminetetraacetic acid , chemistry , nuclear chemistry , metal , metal ions in aqueous solution , microbiology and biotechnology , antibody , biochemistry , chelation , inorganic chemistry , biology , immunology , organic chemistry
Cobalt is a transition metal and an essential trace element that is required for vitamin B 12 biosynthesis, enzyme activation, and so on but is toxic in high concentrations. It was shown that the content of different elements in the plasma of 2‐month‐old BALB/c mice (control group) decreased in the following order: Ca > Mg > Si > Fe > Zn > Cu ≥ Al ≥ B. The treatment of mice with CoCl 2 did not appreciably change the relative content of Ca, Cu, and Zn, but a significant increase in the content of B (2.3‐fold), Mg (1.5‐fold), Al and Fe (2.1‐fold), and Si (3.4‐fold) was found. The treatment of mice led to a 2.2‐fold decrease in the concentration of the total blood protein and a 1.7 ± 0.2‐fold decrease of total immunoglobulin Gs (IgGs). Deoxyribonuclease IgGs corresponding to mice treated (t‐IgGs) and non‐treated (nt‐IgGs) with CoCl 2 contained intrinsically bound metal ions; these IgGs hydrolyzed DNA with very low activity but were not active in the presence of ethylenediaminetetraacetic acid or after Ab dialysis against ethylenediaminetetraacetic acid. The average RAs of deoxyribonuclease nt‐IgGs increased after addition of external metal ions in the following order: Zn 2+ < Ca 2+ < Cu 2+ < Fe 2+ < Mn 2+ < Mg 2+ < Co 2+ < Ni 2+ . Interestingly, t‐IgGs demonstrated lower activities than those for nt‐IgGs either in the absence of external metal ions (2.7‐fold) or in the presence of Cu 2+ (9.5‐fold) > Co 2+ (5.6‐fold) > Zn 2+ (5.1‐fold) > Mg 2+ (4.1‐fold) > Ca 2+ (3.0‐fold) > Fe 2+ (1.3‐fold). However, the RAs of t‐IgGs were remarkably more active than nt‐IgGs in the presence of best activators of t‐IgGs Ni 2+ (1.4‐fold) and especially Mn 2+ (2.2‐fold). The data may be useful for an understanding of Co toxicity, its effect on the concentration of other metal ions, and a change of metal‐dependent specificity of Abzs. Copyright © 2012 John Wiley & Sons, Ltd.