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Probing cytoskeleton organisation of neuroblastoma cells with single‐cell force spectroscopy
Author(s) -
Mescola Andrea,
Vella Serena,
Scotto Marco,
Gavazzo Paola,
Canale Claudio,
Diaspro Alberto,
Pagano Aldo,
Vassalli Massimo
Publication year - 2012
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.2173
Subject(s) - cytoskeleton , biomedicine , microbiology and biotechnology , neuroblastoma , embryonic stem cell , cell , force spectroscopy , cell culture , nanotechnology , biophysics , biology , chemistry , materials science , atomic force microscopy , bioinformatics , genetics , gene
Single‐cell force spectroscopy is an emerging technique in the field of biomedicine because it has proved to be a unique tool to obtain mechanical and functional information on living cells, with force resolution up to single molecular bonds. This technique was applied to the study of the cytoskeleton organisation of neuroblastoma cells, a life‐threatening cancer typically developing during childhood, and the results were interpreted on the basis of reference experiments on human embryonic kidney cell line. An intimate connection emerges among cellular state, cytoskeleton organisation and experimental outcome that can be potentially exploited towards a new method for cancer stadiation of neuroblastoma cells. Copyright © 2012 John Wiley & Sons, Ltd.