Premium
Chemotaxis induced by SXWS tetrapeptides in Tetrahymena —overlapping chemotactic effects of SXWS sequences and their identical amino acids
Author(s) -
Láng Orsolya,
Illyés Eszter,
Menyhárd Dóra K,
Láng Júlia,
Sebestyén Ferenc,
Hudecz Ferenc,
Kőhidai László
Publication year - 2012
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.1166
Subject(s) - chemotaxis , tetrahymena , amino acid , tetrapeptide , chemistry , receptor , stereochemistry , tetrahymena pyriformis , extracellular , biochemistry , residue (chemistry) , peptide
The chemotactic potential of SXWS peptides and the components of the extracellular domain of cytokine receptors were investigated in Tetrahymena as a functional index of substitution with different amino acids in the position ‘X’ of the tetrapeptide. Data obtained demonstrate that position X plays a special determining role in the ligand, SEWS and STWS possess extremely strong chemoattractant ability, and aromatic amino acids result in chemorepellent ligands. Diverse effects of structurally related molecules, for example, SNWS–SDWS, demonstrate a highly sensitive discrimination potential in the applied model system. Physicochemical characteristics (hydropathy, residue size, and solvent‐exposed area) of the amino acids were correlated with the chemotactic activity. Data obtained by computer‐assisted conformation analysis of SXWS peptides and the highly overlapping chemotactic effects of the investigated SXWS peptides as well as the presence of the amino acids in the ‘X’ position indicate that member ‘X’ of the SXWS sequence performs a special role in interactions with the chemotaxis receptors in the membrane. Copyright © 2011 John Wiley & Sons, Ltd.