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Preparation and evaluation of a molecularly imprinted polymer for the selective recognition of testosterone—application to molecularly imprinted sorbent assays
Author(s) -
Tse Sum Bui Bernadette,
Haupt Karsten
Publication year - 2011
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.1162
Subject(s) - molecularly imprinted polymer , molecular imprinting , chemistry , sorbent , selectivity , polymer , combinatorial chemistry , chromatography , aqueous solution , molecular recognition , acetonitrile , imprinting (psychology) , adsorption , organic chemistry , biochemistry , molecule , catalysis , gene
Biomimetic testosterone receptors were synthesized via molecular imprinting for use as antibody mimics in immunoassays. As evaluated by radioligand binding assays, imprinted polymers prepared in acetonitrile were very specific for testosterone because the nonimprinted control polymers bound virtually no radiolabeled testosterone. The polymers present an appreciable affinity, with association constants of K a = 3.3 x 10 7 M − 1 (high‐affinity binding sites). The binding characteristics of the polymers were also evaluated in aqueous environment to study their viabilities as alternatives to antibodies in molecularly imprinted sorbent assays. Compared with the testosterone‐specific antibodies present in commercial kits, our molecularly imprinted polymers are somewhat less sensitive but show a high selectivity. Copyright © 2011 John Wiley & Sons, Ltd.