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In vitro studies with mammalian cell lines and gum arabic‐coated magnetic nanoparticles
Author(s) -
Bicho Ana,
Roque Ana Cecília A.,
Cardoso Ana Sofia,
Domingos Patrícia,
Batalha Íris Luz
Publication year - 2010
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.1066
Subject(s) - nanoparticle , in vitro , adsorption , gum arabic , magnetic nanoparticles , biophysics , iron oxide nanoparticles , cell culture , chemistry , arabic , chemical engineering , nanotechnology , covalent bond , materials science , biochemistry , biology , organic chemistry , genetics , linguistics , philosophy , engineering
Iron oxide magnetic nanoparticles (MNPs) were synthesized by the chemical co‐precipitation method and coated with gum arabic (GA) by physical adsorption and covalent attachment. Cultures of mammalian cell lines (HEK293, CHO and TE671) were grown in the presence of uncoated and GA‐coated MNPs. Cellular growth was followed by optical microscopy in order to assess the proportion of cells with particles, alterations in cellular density and the presence of debris. The in vitro assays demonstrated that cells from different origins are affected differently by the presence of the nanoparticles. Also, the methods followed for GA coating of MNPs endow distinct surface characteristics that probably underlie the observed differences when in contact with the cells. In general, the nanoparticles to which the GA was adsorbed had a smaller ability to attach to the cells' surface and to compromise the viability of the cultures. Copyright © 2010 John Wiley & Sons, Ltd.