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Amino acids–nucleotides biomolecular recognition: from biological occurrence to affinity chromatography
Author(s) -
Sousa F.,
Cruz C.,
Queiroz J. A.
Publication year - 2010
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.1053
Subject(s) - amino acid , nucleotide , molecular recognition , computational biology , chemistry , computer science , artificial intelligence , biochemical engineering , biology , biochemistry , organic chemistry , molecule , engineering , gene
In this review, the protein–DNA interactions are discussed considering different perspectives, and the biological occurrence of this interaction is explained at atomic level. The evaluation of the amino acid–nucleotide recognition has been investigated analysing datasets for predicting the association preferences and the geometry that favours the interaction. Based on this knowledge, an affinity chromatographic method was developed also exploiting this biological favoured contact. In fact, the implementation of this technique brings the possibility to apply the concept of molecular interactions to the development of new purification methodologies. In addition, the integration of the information recovered by all the different perspectives can bring new insights about some biological mechanisms, though not totally clarified. Copyright © 2010 John Wiley & Sons, Ltd.