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Structural prediction and binding analysis of hybridized aptamers
Author(s) -
Zhou Jing,
Soontornworajit Boonchoy,
Snipes Matthew P.,
Wang Yong
Publication year - 2010
Publication title -
journal of molecular recognition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.401
H-Index - 79
eISSN - 1099-1352
pISSN - 0952-3499
DOI - 10.1002/jmr.1034
Subject(s) - aptamer , computational biology , molecular recognition , nanotechnology , biological system , computer science , chemistry , biology , materials science , molecule , microbiology and biotechnology , organic chemistry
Few studies were performed to investigate the molecular recognition capabilities of hybridized aptamers. This study is aimed at applying both theoretical algorithms and experimental assays to examine the effects of hybridization length and region on the secondary structures and binding functionality of hybridized aptamers. The experimental results were significantly different from the structural predictions in many hybridization conditions. To explain this difference, we further proposed a novel equilibrium reaction model that can explicitly analyze the molecular interactions between hybridized aptamers and their targets. We believe that the research findings and the novel model can be used to guide numerous hybridized aptamer‐based applications. Copyright © 2010 John Wiley & Sons, Ltd.