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Transformations of osteoclast phenotype in ia rats cured of congenital osteopetrosis
Author(s) -
Marks Sandy C.,
Schneider Gary B.
Publication year - 1982
Publication title -
journal of morphology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 74
eISSN - 1097-4687
pISSN - 0362-2525
DOI - 10.1002/jmor.1051740203
Subject(s) - osteopetrosis , osteoclast , phenotype , resorption , bone resorption , biology , mutation , mutant , medicine , spleen , endocrinology , genetics , immunology , in vitro , gene
The reduced bone resorption characteristic of osteopetrosis is accompanied in the incisors‐absent ( ia ) rat mutation by a significant increase in osteoclasts of inactive (mutant) phenotype. Restoration of bone resorption in ia rats by transfer of spleen cells from normal littermates is preceded by a transformation of osteoclasts from mutant to normal phenotype. In this investigation the proportions of osteoclasts of normal phenotype have been determined by light microscopy in untreated ia and normal rats and in ia rats treated with various cell populations from normal rats. Significant increases in numbers of osteoclasts of normal phenotype were seen in the mutant skeleton soon after cell treatments that eventually restored bone resorption and cured the disease. No changes in osteoclast phenotype were seen after cell transfers that did not cure the disease. These data establish transformation of osteoclast phenotype as an early event in the recovery from osteopetrosis and suggest that determination of osteoclast phenotype is a reliable predictor of the success of normal cell populations to restore bone resorption in this mutation.