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Consequences of cell death after nitrogen mustard treatment on skeletal pelvic fin morphogenesis in the trout, Salmo gairdneri (Pisces, teleostei)
Author(s) -
Geraudie Jacqueline
Publication year - 1981
Publication title -
journal of morphology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 74
eISSN - 1097-4687
pISSN - 0362-2525
DOI - 10.1002/jmor.1051700205
Subject(s) - biology , anatomy , pelvic girdle , salmo , skeleton (computer programming) , fish fin , mesoderm , nitrogen mustard , epidermis (zoology) , embryonic stem cell , fishery , biochemistry , fish <actinopterygii> , gene , genetics , chemotherapy , cyclophosphamide
Five concentrations of nitrogen mustard (methyl‐bis‐chlorethylamine) have been administrated to different stages of development of trout embryos (Salmo gairdneri) , in order to study their effect on the formation of both pelvic fins soft tissue and skeleton. Sensitivity of epidermis and mesoderm toward this cytotoxic agent has been assessed histologically. Although both components are sensitive to nitrogen mustard, mesoderm is more sensitive than epidermis. These results are compared with the results of similar studies on tetrapod limb buds. The pelvic fin skeleton of some treated animals was doubly stained in toto for cartilage and bone by Alcian blue and alizarin, followed by clearing. Other specimens were processed for histological serial sections. Analysis included comparison of the following parameters: (1) dose of nitrogen mustard, (2) stage of fin bud development at the time of treatment, (3) importance of necrosis in the fin bud, and (4) abnormalities of the endoskeleton of the fin (pelvic girdle and radials) and of the dermal skeleton (lepidotrichia and actinotrichia) one month after the hatching of control animals. Results suggest (1) that material of endoskeleton and lepidotrichia are laid down simultaneously, and (2) that differentiation of actinotrichia is independent of differentiation in endoskeleton and lepidotrichia.