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Ultrastructure of the secretory cells of the submucosal glands in the human maxillary sinus
Author(s) -
Vidić Branislav,
Tandler Bernard
Publication year - 1976
Publication title -
journal of morphology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 74
eISSN - 1097-4687
pISSN - 0362-2525
DOI - 10.1002/jmor.1051500108
Subject(s) - serous fluid , ultrastructure , myoepithelial cell , staining , biology , anatomy , electron microscope , pathology , golgi apparatus , mucous membrane , nasal glands , periodic acid–schiff stain , immunohistochemistry , endoplasmic reticulum , microbiology and biotechnology , biochemistry , mucous membrane of nose , medicine , genetics , physics , optics , immunology
Tissue samples obtained from the lateral wall of the maxillary sinuses of five patients were examined by light microscopical, histochemical, and ultrastructural techniques. Submucosal glands were tubulo‐alveolar mixed glands. The acini consisted of either all serous or all mucous cells, or a mixture of both. Serous granules were stained by toluidine blue, or by hematoxylin and eosin (H and E), but showed little or no reaction with periodic acid‐Schiff (PAS) or Alcian blue. Mucous granules were pale in toluidine blue or H and E preparations, and consisted primarily of acid mucosubstances, as demonstrated by their staining reaction with PAS and Alcian blue. At the electron microscope level, the serous granules were either homogeneously dense, or showed a substructure consisting of at least two layers of distinctly different electron‐opacity. Typical mucous droplets consisted of a fibrillar network dispersed in a translucent matrix. A second secretory product was present in the mucous cells in the form of elongated, membrane‐bounded structures containing numerous parallel filaments, which measured about 55 Å in diameter. The mucous droplets and the filamentous bodies appear to arise from the opposite faces of the Golgi complex in the mucous cells. The filamentous bodies showed a pronounced tendency to fuse with the mucous droplets. All acini were surrounded by a well‐defined myoepithelial layer and contained intercellular nerve terminals.