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Regeneration and supernumerary limb formation under sparsely innervated conditions
Author(s) -
Thornton Charles S.,
Tassava Roy A.
Publication year - 1969
Publication title -
journal of morphology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 74
eISSN - 1097-4687
pISSN - 0362-2525
DOI - 10.1002/jmor.1051270207
Subject(s) - supernumerary , regeneration (biology) , anatomy , biology , transplantation , brachial plexus , larva , medicine , surgery , microbiology and biotechnology , botany
Abstract Normally, urodele limb regeneration is nerve‐dependent. Reduction in nerve‐dependency has been reported for regenerating, transplanted newt limbs (Singer and Mutterperl, '63). Aneurogenic limbs can regenerate without nerves (Yntema, '59). Induction of supernumerary limbs may be obtained from aneurogenic limbs of larval Ambystoma after transplantation orthotopically to innervated larvae and with normal nerve ingrowth to the limb transplant prevented by repeated section of brachial nerves. Of the 13 (of 43) grafts with supernumeraries, nerve counts showed 11 with 0–5; 1 with 5–10; and 1 with 20 + fibers. Orthotopically grafted aneurogenic limbs allowed to become innervated showed 14 supernumeraries in 49 grafts. This supernumerary limb induction is thus not nerve‐dependent. Normally, innervated larval Ambystoma limbs grafted orthotopically and heteroplastically regenerated in 17 of 37 cases after repeated section of brachial nerves. Of the 17 regenerates nerve counts showed 4 with 0–5; 5 with 5–10; 7 with 10–19; and 1 with 20 + fibers. Larval limbs heteroplastically transplanted may require very few or no nerves for regeneration.