Premium
In vitro myogenesis of promuscle cells from the regenerating tail of the lizard, Anolis carolinensis ,
Author(s) -
Cox Prentiss G.
Publication year - 1968
Publication title -
journal of morphology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 74
eISSN - 1097-4687
pISSN - 0362-2525
DOI - 10.1002/jmor.1051260102
Subject(s) - biology , lizard , microbiology and biotechnology , anolis , myogenesis , myocyte , in vitro , population , cell fusion , anatomy , cell culture , zoology , genetics , demography , sociology
Presumptive myoblasts from the regenerating tail of the lizard plated at clonal density undergo extensive growth giving rise to large colonies. After several days in culture at 31°C some of the cells begin to round up and assume a spherical morphology. The number of rounded up cells increases over the next few days until 50 to 75% of the cells are rounded up. If the cultures are switched to a permissive medium, fusion and differentiation occur. If they are left in a nonpermissive medium fusion does not occur. Instead, the cells stretch out on the substrate and become long and attenuated or broad and strap‐like. However, differentiation may continue in some of the cells giving mononucleate cells with cross striations. Autoradiographic studies indicate that the rounded up cells represent a post‐synthesis, prefusion population of cells.