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H 3 ‐proline incorporation into cartilage: Electron microscope autoradiographic observations
Author(s) -
Salpeter M. M.
Publication year - 1968
Publication title -
journal of morphology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 74
eISSN - 1097-4687
pISSN - 0362-2525
DOI - 10.1002/jmor.1051240402
Subject(s) - endoplasmic reticulum , golgi apparatus , cytoplasm , vacuole , biology , microbiology and biotechnology , secretion , cellular compartment , vesicle , extracellular , ground substance , secretory pathway , cartilage , biochemistry , cell , anatomy , membrane , connective tissue , genetics
Electron microscope autoradiography was used to study cartilage from regenerating limbs of adult newts, Triturus, after intraperitoneal injections of proline‐ 3 H. The labeling in the endoplasmic reticulum, small vesicles, Golgi vacuoles, ground cytoplasm and extracellular matrix was compared during the secretion of radioactive products. The data appear to indicate that a large part of the radioactive secretion probably leaves the cell after having been in only one cellular compartment. Although this compartment may be the endoplasmic reticulum, a considerable amount of radioactivity fluxes through the ground cytoplasm and the possibility cannot be excluded that some secretory components leave the cell directly from the ground cytoplasm. The data appear incompatible with the hypothesis that all the radioactivity seen in the extracellular matrix arrived there via a single pathway involving first the endoplasmic reticulum and then the Golgi vacuoles. It is not, however, incompatible with a hypothesis that a fraction of the radioactive product uses this pathway.