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Autoradiographic studies of uridine incorporation in peripheral nerve of the newt, Triturus
Author(s) -
Singer Marcus,
Green Marie R.
Publication year - 1968
Publication title -
journal of morphology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 74
eISSN - 1097-4687
pISSN - 0362-2525
DOI - 10.1002/jmor.1051240306
Subject(s) - axon , biology , rna , myelin , uridine , schwann cell , cytoplasm , myelin sheath , cell bodies , anatomy , cell , microbiology and biotechnology , triturus , biochemistry , endocrinology , central nervous system , gene
Autoradiographic studies combined with digestion tests of incorporated 3 H‐uridine showed that the peripheral nerve of Triturus contains ribonucleic acid. Localization studies revealed the presence of RNA in the axon, in the myelin and Schwann sheath, and in the Schwann cell body. Similar experiments on nerve separated by transection from its neuronal cell bodies yielded the same results. They showed that RNA of the nerve can be synthesized without the intervention of the neuronal cell body. The results strongly suggest that the radioactive substance, precursor or RNA, is transported inward from the Schwann cell to be deposited in the myelin sheath and axon. The route of passage and the possible sites of origin of the RNA in the nerve are discussed. A significant role is suggested for the Schmidt‐Lantermann cleft because of its relations with the adaxonal layer of Schwann cytoplasm and with the myelin leaflets.