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The behavior of the karyosome and the ‘peripheral chromatin’ during mitosis and interkinesis in Amoeba proteus with particular reference to the morphologic distribution of nucleic acid as indicated by the Feulgen reaction
Author(s) -
Chalkley H. W.
Publication year - 1936
Publication title -
journal of morphology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 74
eISSN - 1097-4687
pISSN - 0362-2525
DOI - 10.1002/jmor.1050600103
Subject(s) - feulgen stain , biology , chromatin , nucleic acid , prophase , mitosis , amoeba proteus , haematoxylin , stain , microbiology and biotechnology , biophysics , amoeba (genus) , dna , staining , genetics , meiosis , gene
A comparison of preparations stained with Weigert's haematoxylin and with Feulgen's stain shows that contrary to the commonly accepted view the granules of chromatin (sic.) that lie in interkinesis just beneath the nuclear membrane play no part in the formation of the chromatin of the equatorial plate, but break down during the prophase. They contain at best merely traces of nucleic acid. It is possible that these peripheral granules give rise to, or contribute to, the formation of the spindle fibers and the pole caps. The chromatin granules of the plate arise entirely from the karyosome.