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The effect of fat extraction on Golgi bodies of kidney cells of the frog
Author(s) -
Eastlick Herbert L.
Publication year - 1936
Publication title -
journal of morphology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 74
eISSN - 1097-4687
pISSN - 0362-2525
DOI - 10.1002/jmor.1050590303
Subject(s) - golgi apparatus , biology , kidney , cytoplasm , ground substance , hagfish , potassium permanganate , phospholipid , membrane , microbiology and biotechnology , anatomy , biochemistry , chemistry , connective tissue , endoplasmic reticulum , endocrinology , genetics , organic chemistry , gene , vertebrate
Abstract Frog kidney, when fixed in 4 per cent formalin and subsequently treated with Kolatchev solutions, is found to contain osmiophilic bodies which not only appear identical to the Golgi substance of Kolatchev fixed controls but which also resist bleaching in hydrogen peroxide or potassium permanganate solutions. If a formalin fixed kidney is dehydrated and extracted with various fat solvents and subsequently fixed with the Kolatchev technique, Golgi bodies are usually completely removed but occasionally cells are found which contain spherical globules of osmiophilic substance scattered through the cytoplasm. Kidney tissue fixed in a mixture of 95 parts of alcohol plus 5 parts of formalin before application of the Kolatchev solutions does not contain Golgi bodies. The same results are obtained if benzene is used instead of alcohol and formalin. Striated muscle, when treated in a similar manner, contains darkly stained (Z) and (M) membranes, therefore the osmophilic substance present in muscle quite widely daerent, chemically and physically from that contained in kidney cells. It is apparently an established fact that formalin preserves phospholipids in tissues nnd this investigation has shown that Golgi bodies in kidney cells can be demonstrated following fixation in formalin. Moreover, the Golgi bodies, which are preserved by formalin, can be removed by extracting the tissue with fat solvents. This evidence, therefore, strengthens the hypothesis that true Golgi substance is phospholipid in nature.