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A study of the Golgi elements
Author(s) -
Nahm Laura J.
Publication year - 1933
Publication title -
journal of morphology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 74
eISSN - 1097-4687
pISSN - 0362-2525
DOI - 10.1002/jmor.1050540203
Subject(s) - golgi apparatus , autolysis (biology) , incubation , biology , staining , fixative , incubation period , stain , vital stain , biochemistry , microbiology and biotechnology , cell , cytoplasm , enzyme , genetics
This investigation was undertaken to determine some of the variables involved in the demonstration of the Golgi elements. Gland cells of vertebrates were studied vitally, after impregnation by osmic acid methods, and after autolysis. The duration of the staining period and the concentration of the stain condition the results obtained by vital staining with neutral red. The success of impregnation of Golgi elements with osmic acid varies with,(1) the type of tissue, (2) the time of initial fixation in relation to the time of the death of the animal, (3) the position of the cell in the piece of tissue, and, (4) the temperature of incubation with osmic acid. Stages in the impregnation of Golgi elements can be followed by examining preparations of the same tissue at frequent intervals during the incubation period. An increase in the amount of material which reduces osmic acid and which may form Golgi elements occurs in the cells during autolysis. The results are interpreted as indicating that Golgi elements are visible products of chemical reactions that occur in cells. They represent localized regions containing particular classes of chemical compounds which may be present, as such, in living cells or result from transformations in the cells during incubation. It is suggested that for the cells under consideration these substances may be unsaturated fatty acids.