Cytoplasmic inclusions of Trichamoeba and their reaction to vital dyes and to osmic and silver impregnation

In Trichamoeba sp two types of inclusions are recognized on the basis of reaction to vital dyes and tomethods of osmic and silver impregnation. Globular inclusions, which are stained selectively with neutral red, may be blackened under direct observation by exposure to osmic vapor in hanging‐drop preparations and demonstrated by osmic and silver impregnation. Rod‐like and granular mitochondria, stainable vitally with Janus green, may be distinguished from the neutral‐red globules in preparations stained with a mixture of Janus green and neutral red, and are demonstrated by Regaud's chondriosome method.