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Preparation of deuterated abscisic acid metabolites for use in mass spectrometry and feeding studies
Author(s) -
Zaharia L. Irina,
Galka Marek M.,
Ambrose Stephen J.,
Abrams Suzanne R.
Publication year - 2005
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.939
Subject(s) - chemistry , abscisic acid , chromatography , mass spectrometry , biotransformation , electrospray , tandem mass spectrometry , electrospray mass spectrometry , liquid chromatography–mass spectrometry , deuterium , high performance liquid chromatography , plant hormone , chemical synthesis , organic chemistry , biochemistry , enzyme , in vitro , physics , quantum mechanics , gene
Four deuterium‐labelled ABA catabolites of high isotopic purity were prepared for hormone profiling studies using HPLC‐ESI‐MS‐MS. The catabolites, namely [7′,7′,7′‐ d 3 ]‐PA, [7′,7′,7′‐ d 3 ]‐DPA, [5,8′,8′,8′‐ d 4 ]‐7′‐hydroxyABA and [4,5,8′,8′,8′‐ d 5 ]‐ABA glucose ester were prepared either by biotransformation or chemical synthesis starting with labelled ABA. The labelled compounds are employed as internal standards for plant hormone profiling by liquid chromatography–electrospray–tandem mass spectrometry. Copyright © 2005 John Wiley & Sons, Ltd.