Premium
Convenient synthesis of D ‐ threo ‐[dichloroacetyl 1– 14 C] chloramphenicol
Author(s) -
Sontakke C. V.,
Patil S. P.,
Unny V. K. P.,
Sivaprasad N.
Publication year - 2005
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.932
Subject(s) - chemistry , chloramphenicol , glycine , yield (engineering) , specific activity , chemical synthesis , high performance liquid chromatography , radiochemistry , carbon 14 , chromatography , stereochemistry , nuclear chemistry , organic chemistry , biochemistry , antibiotics , in vitro , amino acid , enzyme , physics , quantum mechanics , materials science , metallurgy
A convenient synthesis of chloramphenicol labelled with carbon‐14 in the dichloroacetyl group at the 1 position is described. It was prepared as part of a 4‐step sequence from [1 ‐ 14 C] glycine and the product was purified by preparative HPLC. A radiochemical yield of 47% was obtained based on [1 ‐ 14 C] glycine and the product had a specific activity of 0.47 mCi/mmol. The procedure can be employed for the synthesis of high specific activity [ 14 C] chloramphenicol, labelled at 1, 2 or both the positions of dichloroacetyl group. Copyright © 2005 John Wiley & Sons, Ltd.