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Enzymatic synthesis of tritium‐labelled isotopomers of L ‐DOPA
Author(s) -
Kozłowska M.,
Kański R.,
Kańska M.
Publication year - 2005
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.919
Subject(s) - isotopomers , chemistry , tritium , enzyme , neurospora crassa , tyrosinase , tyrosine , stereochemistry , radiochemistry , biochemistry , organic chemistry , physics , molecule , nuclear physics , mutant , gene
The synthesis of two isotopomers of L ‐DOPA labelled selectively with tritium is reported. In the intermediate step [3S‐ 3 H]‐, and [3′,5′‐ 3 H 2 ]‐ L ‐tyrosine, have been obtained using a combination of chemical and enzymatic methods. The labelled isotopomers of L ‐tyrosine, L ‐Tyr, have been converted into, [3S‐ 3 H]‐, and [5′‐ 3 H]‐ L ‐DOPA using the enzyme mushroom tyrosinase (monophenol oxidase, EC 1.14.18.1) from Neurospora crassa . Copyright © 2005 John Wiley & Sons, Ltd.