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Enzymatic synthesis of [1‐ 14 C‐ N ‐acetyl, P 18 O 2 ] cytidine monophosphate neuraminic acid
Author(s) -
Burke Erin E.,
Horenstein Nicole A.
Publication year - 2004
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.889
Subject(s) - chemistry , cytidine , pyruvate kinase , neuraminic acid , enzyme , nucleoside , yield (engineering) , uridine , nucleotide , ribonucleoside , stereochemistry , biochemistry , sialic acid , rna , materials science , glycolysis , metallurgy , gene
A method for the enzymatic synthesis of [1‐ 14 C‐ N ‐acetyl, P 18 O 2 ] cytidine monophosphate neuraminic acid (CMP‐NeuAc) is described. Central to the synthesis of [1‐ 14 C‐ N ‐acetyl, P 18 O 2 ]CMP‐NeuAc was the enzymatic preparation of [ γ ‐P 18 O 3 ]ATP for use in a reaction with uridine kinase and cytidine to provide 5′‐[P 18 O 3 ]CMP. The [1‐ 14 C‐ N ‐acetyl, P 18 O 2 ]CMP‐NeuAc isotopomer was then synthesized from a reaction involving nucleoside monophosphate kinase, pyruvate kinase and CMP‐NeuAc synthetase. The isolated reaction yield was 35%. Copyright © 2004 John Wiley & Sons, Ltd.
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