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Red blood cells labelling with 99m Tc‐d,l‐HMPAO: an alternative method for specific cases
Author(s) -
Pérez F.J.,
Matilla A.,
Tercero J.M.,
Martínez F.,
Becerra D.
Publication year - 2003
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.728
Subject(s) - labelling , chemistry , whole blood , in vitro , radiochemistry , nuclear medicine , biochemistry , surgery , medicine
The effectiveness of the classic methods for red blood cells (RBCs) labelling with 99m Tc has been demonstrated in nuclear medicine. However, nuclear physicians have found, in certain circumstances, this diagnostic technique fails and poor quality images are obtained. In this work we report on an alternative method that is of useful in these occasions, for in vitro labelling RBCs with 99m Tc‐d,l‐HMPAO complex. The study shows a high and reproducible labelling efficiency (94.14±0.38), using low amount of tin. The RBCs were isolated from plasma and other interfering blood cells before adding 99m Tc‐d,l‐HMPAO. The tracer was retained and the elution rate from RBCs was low (less than 6% after 120 min). The preclinical results indicate that this new method could be a good alternative to the standard classic methods for specific cases. Copyright © 2003 John Wiley & Sons, Ltd.