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Synthesis of 14 C‐labelled myosmine, [2′‐ 14 C] ‐3‐(1‐pyrrolin‐2‐yl)pyridine
Author(s) -
Tyroller Stefan,
Zwickenpflug Wolfgang,
Richter Elmar
Publication year - 2003
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.678
Subject(s) - chemistry , pyridine , yield (engineering) , sodium hydride , silica gel , chromatography , column chromatography , chemical synthesis , high performance liquid chromatography , organic chemistry , biochemistry , in vitro , materials science , metallurgy
14 C‐Labelled myosmine ([2′‐ 14 C]‐3‐(1‐pyrrolin‐2‐yl)pyridine) was synthesized for autoradiography studies starting from [carboxyl‐ 14 C]‐nicotinic acid by initial esterification of the latter in the presence of 1,1,1‐triethoxyethane. Without any purification the ethyl nicotinate formed was directly reacted with N ‐vinyl‐2‐pyrrolidinone in the presence of sodium hydride, yielding 14 C‐labelled myosmine. The product was purified by silica gel column chromatography. The radiochemical yield was 15% and the specific activity 55.2 mCi/mmol. Copyright © 2003 John Wiley & Sons, Ltd.