Synthesis of  32 P‐labeled intermediates on the purine biosynthetic pathway | Zendy

Mehl Ryan A. | Zendy; Begley Tadhg P. | Zendy

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Synthesis of 32 P‐labeled intermediates on the purine biosynthetic pathway

Author(s) -

Mehl Ryan A.,

Begley Tadhg P.

Publication year - 2002

Publication title -

journal of labelled compounds and radiopharmaceuticals

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.432

H-Index - 47

eISSN - 1099-1344

pISSN - 0362-4803

DOI - 10.1002/jlcr.627

Subject(s) - ribonucleotide , chemistry , ribonucleoside , purine metabolism , purine , stereochemistry , nucleotide , ribonucleotide reductase , adenosine , guanine , carboxamide , purine analogue , biochemistry , enzyme , rna , gene , protein subunit

A fast and efficient route to 32 P‐labeled intermediates on the purine biosynthetic pathway is described. Adenosine kinase catalyzes the phosphorylation of 5‐aminoimidazole‐4‐carboxamide ribonucleoside (AICARs) to give [ 32 P]‐5‐aminoimidazole‐4‐carboxamide ribonucleotide (AICAR). This was then hydrolyzed to [ 32 P]‐4‐carboxy‐5‐aminoimidazole ribonucleotide (CAIR). Treatment of CAIR with N5‐CAIR mutase gave [ 32 P]‐5‐aminoimidazole ribonucleotide (AIR) in milligram quantities. Copyright © 2002 John Wiley & Sons, Ltd.

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