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Synthesis of 32 P‐labeled intermediates on the purine biosynthetic pathway
Author(s) -
Mehl Ryan A.,
Begley Tadhg P.
Publication year - 2002
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.627
Subject(s) - ribonucleotide , chemistry , ribonucleoside , purine metabolism , purine , stereochemistry , nucleotide , ribonucleotide reductase , adenosine , guanine , carboxamide , purine analogue , biochemistry , enzyme , rna , gene , protein subunit
A fast and efficient route to 32 P‐labeled intermediates on the purine biosynthetic pathway is described. Adenosine kinase catalyzes the phosphorylation of 5‐aminoimidazole‐4‐carboxamide ribonucleoside (AICARs) to give [ 32 P]‐5‐aminoimidazole‐4‐carboxamide ribonucleotide (AICAR). This was then hydrolyzed to [ 32 P]‐4‐carboxy‐5‐aminoimidazole ribonucleotide (CAIR). Treatment of CAIR with N5‐CAIR mutase gave [ 32 P]‐5‐aminoimidazole ribonucleotide (AIR) in milligram quantities. Copyright © 2002 John Wiley & Sons, Ltd.