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Increased selectivity in inflammatory site identification via labelling of IgG with N ‐succinimidyl‐4‐[ 125 I]iodobenzoate
Author(s) -
Beiki Davood,
Shahhosseini Soraya,
Khalaj Ali,
Eftekhari Mohammad
Publication year - 2002
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.608
Subject(s) - labelling , chemistry , polyclonal antibodies , chloramine t , in vivo , biochemistry , antibody , immunoglobulin g , chloramine , inflammation , immunology , organic chemistry , biology , microbiology and biotechnology , chlorine
Human nonspecific polyclonal IgG was labelled with 125 I through direct and indirect labelling methods using chloramine‐T and a nonphenolic radioiodinated intermediate N ‐succinimidyl‐4‐[ 125 I]iodobenzoate ( 125 I‐SIB), respectively. Tissue distribution of radioiodinated IgG was assessed in normal and induced inflammation mice. Although, radioiodinated IgG accumulated in the inflammatory area, results showed decreased thyroid and stomach activity and improved inflammatory thigh‐to‐normal tissue ratios with the indirect labelling method ( 125 I‐IB‐IgG) compared with the direct labelling method ( 125 I‐IgG), indicating reduced in vivo deiodination. These results indicate that the 125 I‐SIB is probably a preferable approach for labelling antibodies with iodine radioisotopes. Copyright © 2002 John Wiley & Sons, Ltd.