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Preparation and a simple one‐step purification of [His 1 ‐mono‐ 125 I‐Tyr 10 ,Nle 27 ]‐hGHRH(1‐32)‐NH 2
Author(s) -
Gardi Janos,
Krueger James M.,
Speth Robert C.
Publication year - 2002
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.526
Subject(s) - chemistry , high performance liquid chromatography , elution , chloramine t , chemical synthesis , chromatography , stereochemistry , biochemistry , organic chemistry , in vitro
A one‐step purification of [His 1 ‐mono‐ 125 I‐Tyr 10 ,Nle 27 ]‐hGHRH(1‐32)‐NH 2 , prepared using chloramine‐T, by HPLC with isocratic elution is described. The labeled GHRH analog was suitable for GHRH receptor binding assays. Copyright © 2002 John Wiley & Sons, Ltd.