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Improved specific Synthesis of [1′‐ 15 N]‐ and [3′‐ 15 N] L ‐histidine
Author(s) -
SoedeHuijbregts C.,
van Laren M.,
Hulsbergen F. B.,
Raap J.,
Lugtenburg J.
Publication year - 2001
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.507
Subject(s) - chemistry , histidine , imidazole , labelling , yield (engineering) , stereochemistry , organic chemistry , biochemistry , enzyme , materials science , metallurgy
Abstract Specifically, 15 N‐enriched L ‐histidines have been prepared. The labelling methodology involves introduction of labels in its precursor 1‐benzyl‐5‐hydroxy methyl imidazole, which is converted into L ‐histidine via the Schöllkopf method. The procedure allows the preparation of the intermediates and finally histidine with high 15 N enrichment (99%) at each position, in 29% overall yield starting with 15 NH 4 Cl and 56% with KSC 15 N, respectively. Copyright © 2001 John Wiley & Sons, Ltd.