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Synthesis of multiply labelled ribonucleosides for sequence‐specific labelling of oligo‐RNA
Author(s) -
Milecki Jan,
Földesi Andras,
Fischer Artur,
Adamiak Ryszard W.,
Chattopadhyaya Jyoti
Publication year - 2001
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.503
Subject(s) - chemistry , labelling , rna , ribonucleotide , sequence (biology) , nucleobase , pyrimidine , ribose , stereochemistry , nuclear magnetic resonance spectroscopy , ring (chemistry) , nucleic acid , nucleotide , dna , biochemistry , organic chemistry , enzyme , gene
The synthesis of ribonucleotide blocks multiply labelled with 2 H, 13 C and 15 N for solid support synthesis of sequence specifically labelled RNA is described. Labels were introduced in the ribose ring ( 13 C), C5 position of pyrimidine nucleobases ( 2 H) and exocyclic amino groups ( 15 N) and serve as multiple probes for studying the various physicochemical consequences of physiologically important RNA folding by high‐resolution multi‐nuclear NMR spectroscopy. Copyright © 2001 John Wiley & Sons, Ltd.