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Comparative evaluation of 99m Tc‐ethylene bis‐l‐cysteine and 99m Tc‐ethylene bis‐l‐β‐homocysteine during reversed phase HPLC analysis and electrophoresis at various pH conditions
Author(s) -
Mang'era K.O.,
Tassaert S.,
Cleynhens B.,
Verbruggen A.,
Verbeke K.
Publication year - 2001
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.455
Subject(s) - chemistry , ethylene , high performance liquid chromatography , cysteine , methylene , amine gas treating , electrophoresis , chromatography , reversed phase chromatography , phase (matter) , stereochemistry , medicinal chemistry , organic chemistry , enzyme , catalysis
Ethylene bis‐ L ‐β‐homocysteine ( L,L ‐EH) differs from ethylene bis‐ L ‐cysteine ( L,L ‐EC) in having an extra methylene group between each pair of amine and carboxyl groups. The objective of this study was to determine the effect of the extra methylene groups on the characteristics of the complex of these compounds with technetium‐99m during analysis by reversed phase HPLC and by electrophoresis at various pH values. Up to pH 5.5, 99m Tc‐ L,L ‐EH exhibits a substantially longer retention time during reversed phase HPLC than 99m Tc‐ L,L ‐EC, suggesting a more lipophilic character for 99m Tc‐ L,L ‐EH under these conditions. On the other hand, 99m Tc‐ L,L ‐EH clearly possesses a higher negative charge in the pH range 3‐6.5 as shown by the markedly greater migration towards the anode in electrophoresis experiments. A rational explanation for these seemingly opposing observations can not yet be offered. Copyright © 2001 John Wiley & Sons, Ltd.