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Preparation of [2a,8a,10a,14a‐ 3 H]geldanamycin by biosynthesis
Author(s) -
Taylor George F.,
Foarde Karin K.,
Webber Tricia D.,
Kepler John A.
Publication year - 2001
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.443
Subject(s) - geldanamycin , chemistry , streptomyces hygroscopicus , tritium , specific activity , biosynthesis , catalysis , stereochemistry , chemical synthesis , fermentation , biochemistry , hsp90 , enzyme , in vitro , streptomyces , bacteria , genetics , heat shock protein , physics , biology , nuclear physics , gene
[3‐ 3 H]Propionic acid ( 1 ) was prepared by catalytic hydrogenation of 3‐bromo‐propionic acid with tritium gas. Fermentations of S. hygroscopicus were treated with 1 to produce [2a,8a,10a,14a‐ 3 H]geldanamycin ( 2 ) with a specific activity of 76.2 mCi/mmol. The effect of the amount of 1 used and the dosing pattern on the specific activity of 2 was investigated. Copyright © 2001 John Wiley & Sons, Ltd.