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Development of an automated production process of [ 64 Cu][Cu (ATSM)] for positron emission tomography imaging and theranostic applications
Author(s) -
Liu Tengzhi,
Redalen Kathrine Røe,
Karlsen Morten
Publication year - 2022
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.3973
Subject(s) - positron emission tomography , radiosynthesis , chemistry , radiochemistry , pet imaging , nuclear chemistry , medical physics , nuclear medicine , physics , medicine
Cyclotron‐produced copper‐64 radioisotope tracers offer the possibility to perform both diagnostic investigation by positron emission tomography (PET) and radiotherapy by a theranostic approach with bifunctional chelators. The versatile chemical properties of copper add to the importance of this isotope in medicinal investigation. [ 64 Cu][Cu (ATSM)] has shown to be a viable candidate for imaging of tumor hypoxia; a critical tumor microenvironment characteristic that typically signifies tumor progression and resistance to chemo‐radiotherapy. Various production and radiosynthesis methods of [ 64 Cu][Cu (ATSM)] exist in labs, but usually involved non‐standardized equipment with varying production qualities and may not be easily implemented in wider hospital settings. [ 64 Cu][Cu (ATSM)] was synthesized on a modified GE TRACERlab FXN automated synthesis module. End‐of‐synthesis (EOS) molar activity of [ 64 Cu][Cu (ATSM)] was 2.2–5.5 Ci/μmol (HPLC), 2.2–2.6 Ci/μmol (ATSM‐titration), and 3.0–4.4 Ci/μmol (ICP‐MS). Radiochemical purity was determined to be >99% based on radio‐HPLC. The final product maintained radiochemical purity after 20 h. We demonstrated a simple and feasible process development and quality control protocols for automated cyclotron production and synthesis of [ 64 Cu][Cu (ATSM)] based on commercially distributed standardized synthesis modules suitable for PET imaging and theranostic studies.

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