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Synthesis of [4 S ‐ 2 H]NADH, [4 R ‐ 2 H]NADH, [4‐ 2 H 2 ]NADH and [4‐ 2 H]NAD + cofactors through heterogeneous biocatalysis in heavy water
Author(s) -
Rowbotham Jack S.,
Hardy Adam P.,
Reeve Holly A.,
Vincent Kylie A.
Publication year - 2021
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.3899
Subject(s) - chemistry , cofactor , nad+ kinase , deuterium , nicotinamide , kinetic isotope effect , stereochemistry , nicotinamide adenine dinucleotide , enzyme , biochemistry , physics , quantum mechanics
This practitioner protocol describes the synthesis of a family of deuterated nicotinamide cofactors: [4 S ‐ 2 H]NADH, [4 R ‐ 2 H]NADH, [4‐ 2 H 2 ]NADH and [4‐ 2 H]NAD + . The application of a recently developed H 2 ‐driven heterogeneous biocatalyst enables the cofactors to be prepared with high (>90%) 2 H‐incorporation with 2 H 2 O as the only isotope source.