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Synthesis, bioconjugation and stability studies of [ 18 F]ethenesulfonyl fluoride
Author(s) -
Zhang Bo,
Pascali Giancarlo,
Wyatt Naomi,
Matesic Lidia,
Klenner Mitchell A.,
Sia Tiffany R.,
Guastella Adam J.,
Massi Massimiliano,
Robinson Andrea J.,
Fraser Benjamin H.
Publication year - 2018
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.3667
Subject(s) - chemistry , bioconjugation , conjugate , yield (engineering) , substrate (aquarium) , aqueous solution , combinatorial chemistry , solvent , molecule , amino acid , fluoride , organic chemistry , chromatography , biochemistry , inorganic chemistry , mathematical analysis , materials science , oceanography , mathematics , metallurgy , geology
Fluorine‐18 labelled prosthetic groups (PGs) are often necessary for radiolabelling sensitive biological molecules such as peptides and proteins. Several shortcomings, however, often diminish the final yield of radiotracer. In an attempt to provide higher yielding and operationally efficient tools for radiolabelling biological molecules, we describe herein the first radiochemical synthesis of [ 18 F]ethenesulfonyl fluoride ([ 18 F]ESF) and its Michael conjugation with amino acids and proteins. The synthesis of [ 18 F]ESF was optimised using a microfluidic reactor under both carrier‐added (c.a.) and no‐carrier‐added (n.c.a.) conditions, affording, in a straightforward procedure, 30‐50% radiochemical yield (RCY) for c.a. [ 18 F]ESF and 60‐70% RCY for n.c.a. [ 18 F]ESF. The conjugation reactions were performed at room temperature using 10 mg/mL precursor in aqueous/organic solvent mixtures for 15 min. The radiochemical stability of the final conjugates was evaluated in injectable formulation and rat serum, and resulted strongly substrate dependent and generally poor in rat serum. Therefore, in this work we have optimised a straightforward synthesis of [ 18 F]ESF and its Michael conjugation with model compounds, without requiring chromatographic purification. However, given the general low stability of the final products, further studies will be required for improving conjugate stability, before assessing the use of this PG for PET imaging.