Premium
99m Tc‐labeled oligomeric nanoparticles as potential agents for folate receptor‐positive tumor targeting
Author(s) -
Liang Lili,
Zhang Xueqian,
Su Xiaoyu,
Li Jianchun,
Tian Yong,
Xue Hongbao,
Xu Huiqin
Publication year - 2018
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.3577
Subject(s) - folate receptor , chemistry , conjugate , nanoparticle , in vitro , cysteine , maleimide , covalent bond , receptor , folic acid , radiochemistry , biochemistry , nanotechnology , cancer cell , organic chemistry , enzyme , materials science , medicine , mathematical analysis , mathematics , cancer
One type of biocompatible nanoparticles functionalized with folate and 99m Tc was successfully synthesized. Maleimide‐folic acid (Mal‐FA) was selected to covalently conjugate with ‐SH of the nanoparticles ( NPs ) to prepare NPs‐FA for targeting. 99m Tc was selected to conjugate with ‐NH 2 and ‐SH groups of cysteine residues on the surface of NPs to prepare NPs‐FA‐ 99m Tc for radioactive counting. The ability to target folate receptors of NPs‐FA‐ 99m Tc was assessed in uptake studies with folate‐receptor–positive human HepG2 cells. The results showed that the as‐prepared NPs can selectively uptake by folate receptor‐overexpressing HepG2 tumor cells in vitro. The oligomeric hybrid NPs radiolabeled with 99m Tc may develop to be SPECT/CT imaging biomaterials with high selectivity.