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α v β 3 mediated tumor imaging using 99m Tc labeled NAD/monosaccharide coated ferrihydrite nanoparticles
Author(s) -
Kim EunMi,
Oh PhilSun,
Jeong HwanJeong,
Lim Seok Tae,
Sohn MyungHee
Publication year - 2018
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.3565
Subject(s) - chemistry , ferrihydrite , nad+ kinase , nanoparticle , nuclear chemistry , nicotinamide adenine dinucleotide , glucosamine , radiochemistry , nanotechnology , biochemistry , organic chemistry , enzyme , materials science , adsorption
This study describes the synthesis of highly water‐soluble, non‐toxic, and biocompatible nicotinamide adenine dinucleotide (NAD)/glucosamine (=Nga1Fh) and NAD/glucosamine/gluconic acid coated ferrihydrite nanoparticles (=Nga2Fh) and their possible uses to target tumors in living animals via 99m Tc and 125 I radioisotope labeling. The structural properties were investigated using DLS, zeta potential, TEM, FT‐IR, XRD, and Raman spectroscopy. The cell toxicity in CT26 cancer cells and in vivo tumor targetability in U87MG and CT26 tumor‐bearing mice was further evaluated using cRGDyK‐tagged and cRGDfK‐tagged ferrihydrite nanoparticles. The average diameters of the resulting Nga1Fh and Nga2Fh nanoparticles were <5 to 7 and <3 nm, respectively. The Nga2Fh nanoparticles did not show cell toxicity until 0.1 mg/mL. Using gamma camera imaging, 99m Tc‐cRGDfK‐Nga2Fh showed the highest tumor uptake in a U87MG tumor‐bearing mouse when compared with that of 99m Tc‐cRGDyK‐Nga2Fh and 99m Tc‐Nga2Fh. The image‐based tumor‐to‐muscle ratio by time for 99m Tc‐cRGDfK‐Nga2Fh was 3.8 ± 1.7, 4.2 ± 2.0, 7 ± 1.5, 13 ± 2.0, 8 ± 3.7, and 2 ± 1.6 at 5 and 30 minutes, 1, 2, 4, and 24 hours, respectively. Although further studies are needed, the NAD/monosaccharide coated ferrihydrite nanoparticles could be presented as an interesting material for a drug delivery system.