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Stable and high‐yielding intrinsic 59 Fe‐radiolabeling of the intravenous iron preparations Monofer and Cosmofer
Author(s) -
Jensen Andreas I.,
Severin Gregory W.,
Andreasen Hans B.,
Rasmussen Palle H.
Publication year - 2016
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.3416
Subject(s) - chemistry , size exclusion chromatography , elution , radiochemistry , aqueous solution , in vivo , absorption (acoustics) , matrix (chemical analysis) , chromatography , specific activity , nuclear chemistry , biochemistry , organic chemistry , physics , microbiology and biotechnology , acoustics , biology , enzyme
Commercial iron supplements Monofer ® and Cosmofer ® were intrinsically radiolabeled with 59 Fe for the purpose of tracing iron absorption in vivo . Optimized procedures aimed at introducing 59 Fe into the macromolecular construct in a form that was as chemically equivalent to the matrix iron as possible. This was determined by challenging the labeled constructs with diethylenetriaminepentaacetic acid (DTPA) followed by separation by size‐exclusion and measurements of radioactivity and iron in the eluted fractions. The final procedures were simple and involved heating aqueous dispersions of the supplements in the presence of [ 59 Fe]FeCl 3 for 24 h at 95 °C for Monofer, and 85 °C for Cosmofer, resulting in radiochemical yields greater than 94%. High performance size exclusion chromatography, UV‐VIS spectroscopy, and dynamic light scattering were used to show that the supplements remained unchanged after radiolabeling, underscoring the applicability of the methodology for radiolabeling commercial iron preparations.