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A study of radiogallium aqueous chemistry: in vitro and in vivo characterisation of 67 Ga‐hydrolysed‐stannous fluoride particles
Author(s) -
Tsopelas Chris
Publication year - 2016
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.3388
Subject(s) - chemistry , in vivo , radiochemistry , fluoride , biodistribution , aqueous solution , in vitro , hydrolysis , colloid , nuclear chemistry , particle size , chloride , chromatography , inorganic chemistry , biochemistry , organic chemistry , microbiology and biotechnology , biology
The objective of this study was to explore the aqueous chemistry of gallium using 67 Ga‐chloride starting material, by radiolabelling hydrolysed( h )‐stannous fluoride particles and then characterising the optimal formulation for radiochemical purity (RCP) and radioactive particle size distribution in vitro . The pilot reactions determined stannous fluoride was added to 67 Ga‐acetate under nitrogen and then heated at 100 °C for 20 min to achieve ≥95% RCP and 67 Ga‐particles were >3 µm in diameter. A high radioactive concentration of 67 Ga‐ h ‐SnF 2 particles could be prepared similarly in ≥97% RCP with 74% as 3–5 µm and 26% >5 µm in diameter. The latter formulation had larger particles than 99m Tc‐ h ‐SnF 2 colloid (96% of 1–3 µm), and it resulted in a rat biodistribution of 41% in the lungs, 41% in the liver plus spleen and 18% in the carcass at 20 min after injection. The carcass activity was attributed to bone marrow and some 67 Ga‐transferrin formed in blood. Isolated mixed human leucocytes were radiolabelled with 67 Ga‐ h ‐SnF 2 particles in 100% efficiency, and the 67 Ga‐cells did not release soluble 67 Ga 3+ at room temperature over 3 h. The 67 Ga‐ h ‐SnF 2 particle formulation could find a use in labelling leucocyte cells for in vivo homing studies when delayed animal imaging is required.