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Gamma scintigraphy and biodistribution of 99m Tc‐cefotaxime sodium in preclinical models of bacterial infection and sterile inflammation
Author(s) -
IlemOzdemir Derya,
Asikoglu Makbule,
Ozkilic Hayal,
Yilmaz Ferda,
HosgorLimoncu Mine,
Ayhan Semin
Publication year - 2016
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.3374
Subject(s) - biodistribution , chemistry , cefotaxime , radiochemistry , inflammation , technetium , technetium 99m , scintigraphy , nuclear medicine , nuclear chemistry , antibiotics , medicine , biochemistry , in vitro
99m Tc‐cefotaxime sodium ( 99m Tc‐CEF) was developed and standardized under varying conditions of reducing and antioxidant agent concentration, pH, radioactivity dose, and reducing agent type. Labeling studies were performed by changing the selected parameters one by one, and optimum labeling conditions were determined. After observing the conditions for maximum labeling efficiency and stability, lyophilized freeze dry kits were prepared accordingly. Simple method for radiolabeling of CEF with 99m Tc has been developed and standardized. Labeling efficiency of 99m Tc‐CEF was assessed by both radio thin‐layer chromatography and radio high‐performance liquid chromatography and found higher than 90%. The labeled compound was found to be stable in saline and human serum up to 24 h. Two different freeze dry kits were developed and evaluated. Based on the data obtained from this study, both products were stable for 6 months with high labeling efficiency. The prepared cold kit was found sterile and pyrogen free. The bacterial infection and sterile inflammation imaging capacity of 99m Tc‐CEF was evaluated. Based on the in vivo studies, 99m Tc‐CEF has higher uptake in infected and inflamed thigh muscle than healthy thigh muscle.