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A new 68 Ga anionic concentration and purification method for automated synthesis of [ 68 Ga]‐DOTA or NODAGA conjugated peptides in high radiochemical purity
Author(s) -
Ben Azzouna Rana,
Alshoukr Faisal,
Leygnac Sébastien,
Guez Alexandre,
Gonzalez Walter,
Rousseaux Olivier,
Guilloteau Denis,
Le Guludec Dominique
Publication year - 2015
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.3316
Subject(s) - chemistry , labelling , yield (engineering) , elution , radiochemistry , chromatography , dota , specific activity , nuclear chemistry , chelation , inorganic chemistry , enzyme , organic chemistry , biochemistry , materials science , metallurgy
The 68 Ge/ 68 Ga generator is of increasing interest for clinical PET. For successful labelling, the eluate has to be purified. The aim of our approach is to improve the existing anionic methods which have a number of advantages compared to other methods but which use high concentrated HCl, and require an additional anionizing step. A new 68 Ga‐eluate anionic purification method that enables rapid and high efficiency labelling of DOTA and NODAGA conjugated peptides in high radiochemical purity is described. The new method uses NaCl as an alternative Cl − source to the corrosive HCl and combines the three standard steps in a single step. The recovery yield was ≥90%, and the 68 Ge breakthrough was in conformity with the European Pharmacopeia limit. An automated labelling of DOTA and NODAGA‐conjugated peptides was performed with the new method, using acetate sodium buffer, with a total duration of 13 min and a radiochemical yield >85%. The labelled peptides have a radiochemical purity exceeding 99% and can be used directly without any further purification step and without the quality control by gas chromatography. Furthermore, the new method has an economic advantage: it offers the possibility to use generator until 20 months after the calibration date.