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Fully automated synthesis and coupling of [ 18 F]FBEM to glutathione using the iPHASE FlexLab module
Author(s) -
Ackermann Uwe,
Plougastel Lucie,
Wichmann Christian,
Goh Yit Wooi,
Yeoh Shinn Dee,
Poniger Stan S.,
TochonDanguy Henri J.,
Scott Andrew M.
Publication year - 2014
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.3175
Subject(s) - chemistry , glutathione , specific activity , conjugated system , thiol , high performance liquid chromatography , macromolecule , combinatorial chemistry , radiochemistry , chromatography , biochemistry , organic chemistry , polymer , enzyme
Site‐specific radiolabelling of peptides or antibodies using [ 18 F]FBEM is often preferred over non‐site‐specific radiolabelling with [ 18 F]SFB because it does not affect the affinity of the antibody to its target. Unfortunately, the synthesis of [ 18 F]FBEM and its conjugation to thiol containing macromolecules requires some manual intervention, which leads to radiation exposure of the radiochemist. In this publication, we report on the complete automation of [ 18 F]FBEM production and its subsequent conjugation to glutathione using a slightly modified iPHASE FlexLab module. [ 18 F]FBEM was produced in 1.185 ± 0.168 GBq (15–20%; n = 10; 0.75 ± 0.106 GBq non‐decay corrected) with a specific activity of 57 ± 10 GBq/µmol. Radiochemical purity was 97 ± 1% and the synthesis time including HPLC purification and reformulation was 70 min. After evaporation to dryness, [ 18 F]FBEM was conjugated to glutathione in PBS buffer pH 7.4 in quantitative yields. This fully automated method does not require any manual intervention and therefore reduces the radiation exposure to the operator.