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The synthesis of a carbon‐14 labeled pegylated Adnectin™ for placental transfer studies in guinea pigs
Author(s) -
Maxwell Brad D.,
Cao Kai,
Bonacorsi Samuel,
Sivaraman Lakshmi,
Wang Bonnie,
McNerney MaryEllen,
Wang Lifei,
Christopher Lisa J.,
Fung Nga Kit Eliza,
Wang Jian,
Arnold Mark E.
Publication year - 2013
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.3077
Subject(s) - maleimide , chemistry , linker , polyethylene glycol , monoclonal antibody , peg ratio , biochemistry , molecular mass , antibody , immunology , organic chemistry , enzyme , operating system , finance , computer science , economics , biology
Adnectins™ are novel fibronectin‐based proteins containing domains engineered to bind to targets of therapeutic interest. The molecular weights of adnectins are less than conventional monoclonal antibodies but larger than traditional small molecules. Until now, there has been no information on the placental transfer of adnectins. To assess placental permeability to adnectins in pregnant guinea pigs, a radiolabeled adnectin, ATI‐1072, bound to polyethylene glycol through a [ 14 C]Maleimide linker, was synthesized from [1,4‐ 14 C]Maleic acid. This publication describes the synthesis and analysis of PEG‐[ 14 C]Maleimide‐adnectin ([ 14 C]ATI‐1072). Copyright © 2013 John Wiley & Sons, Ltd.